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KMID : 0191120150300070866
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Journal of Korean Medical Science
2015 Volume.30 No. 7 p.866 ~ p.870
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Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi
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Lee Sun-Myoung
Kwon Hea-Yoon
Im Jae-Hyong
Baek Ji-Hyeon
Kang Jae-Seung
Lee Jin-Soo
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Abstract
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Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to the survival of the pathogen. However, there have been no reports confirming whether O. tsutsugamushi indeed produce OMVs. O. tsutsugamushi boryong was cultured in ECV-304 cells for the purification of OMVs. Western blot analysis and immunoenrichment using anti-O. tsutsugamushi monoclonal antibody and electron microscopy were employed for identification and characterization of OMVs. We confirm the presence of OMVs derived from O. tsutsugamushi, and also found that those OMVs contain a major surface antigen of 56-kDa protein and variant immunogenic antigens.
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KEYWORD
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Orientia tsutsugamushi, Scrub Typhus, Outer Membrane Vesicles, Antigens
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